SBC's Extraction laboratory is the first stop for dried plant samples that are collected from the field collection and from the local communities. All the plant samples will be processed at the Plant Preparation area. The process includes washing, sorting, cutting, drying and grinding the plant samples. The extraction of ground plant samples will be carried out in the extraction laboratory.
In the extraction laboratory, plant samples are extracted using various methods such as maceration solvent extraction, maceration aqueous extraction, liquid-liquid partition, and reflux extraction.
The plant extraction process is monitored through the Laboratory Information Management System (LIMS), and barcodes are generated from LIMS to act as the security code for the database. These plant extracts are then deposited into the Natural Product Library.
The protocol for routine extraction is based on the NCI (National Cancer Institute) protocol. The extraction methodology is as follows:
- Plant Samples (Ground Plant Samples)
- Soak in methanol: dichloromethane in ratio 1:1 for 24 hours
- Concentrated using rotary evaporator
- Crude extracts